Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples

Though many persistent organic pollutants (POPs) are closely regulated the human population is still exposed to these ubiquitous chemicals from environment and diet. Safe management biomonitoring of POPs necessary understand risk exposure. Within mass sample often limited, therefore robust methods using smaller amounts necessary. This study developed a 96-well plate solid phase extraction (SPE) method for determination selected POPs: polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD) non-persistent novel flame retardants (NFRs) in low volume blood serum. Non-destructive clean-up coupling Oasis HLB with Phree phospholipid removal was employed. Extraction efficiency determined at high concentrations certified reference materials NIST SRM 1957 1958, respectively. Target compounds deviated values on average by 15% 21% Observed limit detections (LODs) ranged 0.36 pg/mL (PCB 180) 66.07 (?-HCH). The applicability real samples demonstrated 48 pregnant women enrolled pilot CELSPAC: TNG study. In total, 30 target were detected least one sample. here provides fast reliable analysis serum possibility introduce automation preparation procedure.